Facts About attune flow cytometer Revealed
Facts About attune flow cytometer Revealed
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Details-enriched characterization of cell populations. Morphological data from photographs can incorporate into the richness of flow cytometry details. As an example, the figure exhibits an if not traditional apoptosis assay utilizing Annexin V and PI, including mobile imaging to characterize cells in Each individual population to expose morphologically distinctive attributes. These insights could not are already acquired from multiplex staining on your own.
No matter whether you’re a newbie or a specialist, discover our means to progress your study and abilities in flow cytometry.
Incorporate acoustic concentrating technology with superior-parameter Evaluation for versatile spectral unmixing and standard workflows.
Discover new insights into your experimental data by visualizing populations working with put together fluorescence and morphology info. Our program interface wizards permit downsampling, concatenation (merged files) and dimensionality tools which have been built in to the software program for an intuitive, streamlined data Assessment expertise.
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Below, a skilled person has gated singlets confidently. Following assessing the manual singlet gate, the CytPix impression-derived parameter ParticleCount reveals this gate includes greater than 4% aggregates.
Optimizing gating tactic. Even strong manual singlet gating is error-prone and stays a subjective selection place in Practically all flow cytometry assays. Imaging may be used to verify and regulate gates to incorporate only one cells of interest.
Steady impression high-quality even at higher throughput Acoustic concentrating and also a significant-pace camera Mix to impression these T cells consistently at minimal or significant flow fees. Quickly change aim and digital camera options to fulfill experimental prerequisites.
Early indications of this contamination experienced originally been dismissed as debris. Backgating to discover new populations. Backgating imaged cells about the Attune CytPix Flow Cytometer also lets you use morphological attributes to find out fascinating subpopulations that get more info might not be evident from flow cytometry information alone.
Potentially most importantly, these gatherings comprise cells of Plainly unique phenotypes which could lead on to incorrect conclusions with regards to double favourable gatherings (especially in exceptional populations).
AI-driven automatic picture Investigation While using the Attune Cytometric Computer software interprets event options into distinct morphology parameters that can be combined with typical fluorescence and scatter parameters to reveal very reproducible population changes and exclusive subsets.
Software Be aware: Automatic graphic Examination reduces person-to-person variability in flow cytometry gating tactics
Leverage the strength of Highly developed technology and innovation to accelerate your discoveries. Have confidence in within the Attune Flow Cytometer family to provide the trustworthiness, velocity, and precision that set your get the job done apart.
Versatile payment plan from Agilent Monetary Options permit you to have the significant instrumentation you require for fast task, or to develop your lab.
Gating on these prolonged parameters enables you to rapidly and precisely recognize populations of desire and confirm gating approach with little if any guide evaluate. The Attune software delivers use of condition-of-the-artwork equipment Studying Evaluation instruments in an expertly developed user interface, rendering it extremely simple to operate.
Because the cell line had Formerly been analyzed on the Attune CytPix Flow Cytometer, the researchers went back to the images and were being capable of document the microbial infection at least five days previously.
Agilent Benefit Guarantee guarantees you a minimum of ten years of instrument use from your date you purchase. In any other case, We'll credit rating you With all the residual value of the method towards an upgraded design.
Here, we clearly show that by gating methods working with image-based mostly quantitative parameters can distinguish interacting cells from coincident functions to much more accurately evaluate interacting cells.